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Abstract Glass Building

*Ex Vivo Calcium Imaging using Brain-on-Chip Microfluidics
Formal training and implementation of ex vivo microfluidics of the suprachiasmatic nucleus of the hypothalamus on mice. 

*Advanced Microscopy
Designed and built a high-resolution, laser Leica D4M B microscope. Experience imaging and z stacking immunofluorescence/bright-field neural tissue for colocalization analysis. 

*3D CAD modeling experience with AutoDesk inventor
Generated technical drawings using a computer aided drafting system (AutoCAD). 

*R and Python-based bioinformatics/data analysis
Expertise in principal component analysis and clustering analysis (k means, agglomerative and divisive) on large datasets.  
 
*Supervised and unsupervised machine learning (using R and python-based methods)
Extensive experience using various machine learning models, including decision tree, random forest, boost, support vector machine, linear, and neural net machine learning.
 
*Behavioral testing
Expertise in independently performing various rodent behavioral tests, including parental (alloparental) care, social interaction, partner preference, elevated zero maze, open-field, condition-placed preference, and self-administration.

*Next-generation RNA sequencing (via laser-capture microdissection)
Expertise in extracting RNA from flash frozen slides by targeting region-specific neuronal populations using Leica’s LMD6500 laser capture microdissection scope.
 
*Immunohistochemistry
Expertise in performing colocalization staining both for bright-field and immunofluorescence assays. 

*Genomic analysis using Restriction site-associated DNA sequencing 
Expertise in bioinformatic analysis using various python scripts and r platforms. This includes obtaining raw illumina ddRAD-seq data and process it for population genomics.

*fMRI and DWI preprocessing and processing using FSL, ANTS, AFNI and SPM
Expertise analyzing data derived from neural functional magnetic resonance imaging. 
 
*Graph theory network analysis via Gephi software
Expertise in creating network models and performing various degrees of statistical analysis of global and nodal centralities.

*Stereology (via MBF stereo investigator software)
Extensive experience in three-dimensional cell counting using the optical fractionator probe.
 
*R and Prism-based data analysis and figure production
Extensive experience in performing various statistical analysis and figure production using Prism and R programming tools.

*Proficient in Microsoft Office Suite (Word, Excel, Access, and PowerPoint)

*Proficient in adobe illustrator 
View journal cover art publications:

*Communication
Communicated research to the broader public as well as at numerous scientific meetings and conferences.

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