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*Ex Vivo Calcium Imaging using Brain-on-Chip Microfluidics
Formal training and implementation of ex vivo microfluidics of the suprachiasmatic nucleus of the hypothalamus on mice.
*Advanced Microscopy
Designed and built a high-resolution, laser Leica D4M B microscope. Experience imaging and z stacking immunofluorescence/bright-field neural tissue for colocalization analysis.
*3D CAD modeling experience with AutoDesk inventor
Generated technical drawings using a computer aided drafting system (AutoCAD).
*R and Python-based bioinformatics/data analysis
Expertise in principal component analysis and clustering analysis (k means, agglomerative and divisive) on large datasets.
*Supervised and unsupervised machine learning (using R and python-based methods)
Extensive experience using various machine learning models, including decision tree, random forest, boost, support vector machine, linear, and neural net machine learning.
*Behavioral testing
Expertise in independently performing various rodent behavioral tests, including parental (alloparental) care, social interaction, partner preference, elevated zero maze, open-field, condition-placed preference, and self-administration.
*Next-generation RNA sequencing (via laser-capture microdissection)
Expertise in extracting RNA from flash frozen slides by targeting region-specific neuronal populations using Leica’s LMD6500 laser capture microdissection scope.
*Immunohistochemistry
Expertise in performing colocalization staining both for bright-field and immunofluorescence assays.
*Genomic analysis using Restriction site-associated DNA sequencing
Expertise in bioinformatic analysis using various python scripts and r platforms. This includes obtaining raw illumina ddRAD-seq data and process it for population genomics.
*fMRI and DWI preprocessing and processing using FSL, ANTS, AFNI and SPM
Expertise analyzing data derived from neural functional magnetic resonance imaging.
*Graph theory network analysis via Gephi software
Expertise in creating network models and performing various degrees of statistical analysis of global and nodal centralities.
*Stereology (via MBF stereo investigator software)
Extensive experience in three-dimensional cell counting using the optical fractionator probe.
*R and Prism-based data analysis and figure production
Extensive experience in performing various statistical analysis and figure production using Prism and R programming tools.
*Proficient in Microsoft Office Suite (Word, Excel, Access, and PowerPoint)
*Proficient in adobe illustrator
View journal cover art publications:
*Communication
Communicated research to the broader public as well as at numerous scientific meetings and conferences.
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